Journal: Cancer Medicine

Article Title: An antibody‐drug conjugate targeting a GSTA glycosite‐signature epitope of MUC1 expressed by non‐small cell lung cancer

doi: 10.1002/cam4.3554

Figure Lengend Snippet: Staining of non‐small cell lung cancer (NSCLC) cells and tissues by 16A (specific to GSTA neoantigen), SM3, and C595 (specific to PDTR neoantigen). (A) NSCLC cell lines were stained with 16A, SM3, and C595 antibodies. (B) Tissue array slides containing consecutive tissue sections from the same NSCLC patients were stained with 16A, SM3, and C595 antibodies.

Article Snippet: Tumor tissue array slides were obtained from CrownBio.

Techniques: Staining

Journal: Cancer Medicine

Article Title: An antibody‐drug conjugate targeting a GSTA glycosite‐signature epitope of MUC1 expressed by non‐small cell lung cancer

doi: 10.1002/cam4.3554

Figure Lengend Snippet: Expression of the aberrantly glycosylated MUC1 peptide motif in lung and breast cancer tissues upon 16A antibody staining. (A) H‐score of the cancer tissue array stained with the 16A antibody. The intensity of IHC staining was scored at four levels: 0 (negative), 1 (weak staining), 2 (medium staining), and 3 (strong staining). The percentages of tumor cells at different intensity levels were evaluated. Total Score = (% at 0) ×0 + (% at 1) × 1 + (% at 2) ×2 + (% at 3) × 3. (B) 16A positivity in lung cancer, breast cancer, and triple‐negative breast cancer (TNBC) samples. (C, D, E, F) Representative photographs of MUC1 immunostaining in peritumoral (C), lung cancer (D), Non‐TNBC (E), and TNBC (F) tissue with the 16A antibody (original magnification ×200). Positivity was defined as ≥30% of tumor with staining ≥2+.

Article Snippet: Tumor tissue array slides were obtained from CrownBio.

Techniques: Expressing, Staining, Immunohistochemistry, Immunostaining